Get it as soon as Fri, Aug 19. View Varients. The body temperature during all surgical procedures and during recovery from anesthesia is maintained using a heat lamp and/or heating pad. Heat the mouse with an infrared lamp. Intravenous (IV) "Lateral Tail Vein Rat" Supplies: * 1-3ml syringe * 25g Needle * Heat Source Max Volume 0.5ml. . To facilitate tail vein visualization and ensure optimal injections, dilate the tail vessels immediately prior to injection by warming the tail of the mouse with a safe, effective heat source (e.g. If injection of unanesthetized animals is required, the animal is placed in an appropriate passive restraint device (e.g. 2. Briefly, mice were warmed for 5 min using a heat lamp and a heating pad, and then siRNA expression plasmid (pSilencer4.1/Sry565) or negative control plasmid (pSilencer4.1/EGFP) was mixed with or without PEI micelles . (15 to 25 cm) above the . it is kept warm by a heat lamp or other heating device (e.g., Deltaphase pads). veins more apparent- wipe in the direction of the scales to avoid irritation to the tail. Preparation for the surgeon: The surgeon wears a facemask, hair bonnet, surgical gown and clean . As the mouse tail warms up, the vein should dilate and become more visible. The veins of the tail should be easily visible. 3. Use the smallest gauge needle you can get,. Tumors and livers via were collected 13 h after administration. This protocol details the fabrication and application of a 3D-printed device for facilitating lateral tail-vein injection in mice. The injector then uses their other hand to advance a 28 or 30 G needle into the mouse tail vein until a flashback of blood is observed. The more 72 acceptable alternative is to dilate the veins by immersing into warm water (40 to 45 C) (8). Figure 13: Intramuscular Injection Site Figure 14: Intramuscular Injection - 2 people Intramuscular Injection - Restrainer Or transplant the stem cells by retro-orbital injection (easier at the beginning). Unlike the tail vein route, the femoral vein cut down requires the use of anesthesia. Disinfect the site of injection and slightly rotate the tail to visualize the vein. Tail Vein Injections in the Mouse and Rat SOP Page 3 of 3 5. You can inject up to 400 ul in volume. 73 74 Tail vein injection also requires the animal to be mechanically restrained, and this can cause The rodents are initially put under a heat lamp to increase blood flow to the tail vein. IV vascular access is technically challenging in the adult mouse. The depth of anesthesia is monitored via withdrawal response and respiratory rate. In most cases warming the tail with the aid of a heat lamp or warm compresses will increase obtainable . - 0.5 mL syringe with 27-G ( 1/2-in.) Lateral tail vein injection of mice for Mtb infection (A) The tail of the mouse can be warmed using methods such as a light source, warm water, or a red heat lamp for vasodilation and ease of. 7th Oct, 2013 Alexa Wu Claude Bernard. The mouse, Mus musculus, is the most commonly used laboratory animal.One of the most common and efficient routes of administration or injection in mice is the intravenous (IV) route. More then one animal can be heated at a time. via tail vein slowly (another 2-3 minutes). CTKcom UVA UVB Light Bulb Reptile Ceramic Heat Lamp Pet Heating Bulb Holder Clamp Lamp Fixture Heating Light Lamp For Reptiles,Aquarium Reptile Light Adjustable Habitat Lighting Stand,110V-130V(Black) 4.0 out of 5 stars 433. For better visualization of the vein and dilation, warm up the vein to ~37C using a water bath or a heat lamp. vii. a. vi. Mice were maintained under isoflurane anesthesia during the injection, accumulation, and scanning periods. Mice received either retro-orbital or tail-vein injections daily for 5 d. On the day after the final injection, we killed. Once the tail is heated to adequate temperature, the animal is ready for the procedure. 3. You can use commercially available re-usable "Hot Button- hot pack" that instantly becomes hot once the button inside the pack is activated. Transfer mice to a restrainer with a hole for the tail. Histology and immunohistochemistry. Isolate the mouse in a small cage or container. When anesthesized (ketamine/xylazine or isoflurane) we keep our mice on a heated pad anyways. Do not keep mice in the heat box for more than 20 min. The heat lamp poses a high 71 risk of causing hyperthermia, heat distress and burn-related injuries to the animals. Sliding doors keep mouse gently restrained. Be careful of the length of time the mouse is left under the lamp. CAUTION: do not overheat; animal should be under a heat lamp no longer than three minutes with heat lamp at least 12 inches away. The tail may need to be washed with diluted Hibiscrub (1%) in order to see the blood vessel. If there is resistance upon pushing the plunger, the needle is not placed properly into the vein. Intravenous Tail Vein Injection Needle size 25 - 30G x 13mm, maximum bolus volume 1% of animal's body weight. Swab the tail with 70% ethanol on a gauze sponge or swab. . Position the heat lamp at an appropriate distance from the animal cage to provide warmth and vasodilation. Gene Expression in the Liver and in Tumors Wrap tail in an exam glove filled with warm water for 12 minutes iii. 2.4. Some technicians place animals under heat lamps, but this is discouraged, especially in small rodents, as the animals are often overheated leading to additional trauma and discomfort. 3. Warm the tail by immersing it in warm water or placing the animal under a heat lamp. Rodent Tail Vein Injections (Mice) Date Issued: July 7, 2011 Revision: 2 Page No: 4 . 2. Live animal imaging was done on the IVIS 200 machine (Caliper Life Sciences . Must inject slowly . Tail Vein Injection 1. Mice were main-tained under isourane anesthesia during the injection, accumu-lation, and scanning periods. 1. with a little modification. Techniques for tail vein dilation: The following techniques may be used to increase blood flow on the tail vein of a mouse or a rat: 1) Use of a heating lamp* 2) Submerging the tail in warm water (no warmer than 40 o C/104 o F) * 3) Placing rubbing alcohol over the tail * Animals under a heat lamp must be under direct supervision and care must . The tail vein is easier to see in non-pigmented rats. Place mouse in a restrainer. Introduce a 27G syringe into tail vein to administer an injectate into mice. through the tail vein. Note: Heat lamps and heated surgery pads may also be used to induce tail vein dilatation. Hydrodynamic Injection: 1. Fish: 0.0025-0.01% (25-100mg/L) solution. between 3-8 separate litters. The lateral (side) veins are immediately below the skin, and must be . Heat lamps or heated surgery pads, while not necessary, will also help by increasing the volume of the tail vein. Heat Lamp with table clamp. Use heat to dilate the tail veins which run laterally along each side of the tail. Some crazy procedures allow you to do 1.5 ml. Cleaning the tail with ethanol is of some help and also, as you said, warming the tail. The Mouse Tail Illuminator efficiently illuminates and warms a mouse tail for easy injection or bleeding. 0.05-0.2% (500-2000mg/L) solution. Six hours was chosen to allow for the . Warm the tail with a heat lamp or by immersing in warm water to dilate vessels. 2. Cannulation in the tail does not sound like a good idea to me, especially . Take mouse out of strainer and check for vitality signs (normal heartbeat and breathing). We are a women owned small business, dedicated to providing new product technology and supplies to advance medical milestones for all. the left and right lateral tail veins, in the ~10 and 2 o'clock positions (Figure 18). vector per mouse in sterile PBS or DMEM via tail vein. This will increase the blood supply to the tail, enlarging the veins and making them easier to locate and inject. . (C) Anesthetizing the mouse with ketamine. A fine gauge needle should be used for this procedure. Intravenous injections Intravenous injections are usually made into the dorsal tail vein. Hydrodynamic tail vein injection]. 3. 1. Restraining tube slides off base for easy cleaning. You should be able to see the vein blanch if the needle is properly positioned. 99. Intravenous Injections Warm the mouse under a heat lamp or other heating device, being sure not to OVERHEAT the animal. 4. ,MSPP-TV-150STEA,MSPP-TV-150LGEA Soft Temp Reusable Veterinary Pads, Braintree Scientific BN-LINK Digital Heat Mat Thermostat Controller for Seed Germination, Reptiles and Brewing Breeding . Tail-Vein Injection . A second scan was performed with a repeat injection of 18 F-FDG approximately 6 h after the first injection. Injections via the retro-orbital vascular sinus do not depend . Power: 110VAC Bulb: 250 watt infrared heat Lamp Infrared heat lamp is table mounted with an adjustable swing arm providing for easy adjustment of radiant heat to specimen. dynamic Tail Vein Injectiong pDNA-coding LacZ (2 pDNA/2 mL saline/mouse) was administered into tumor-bearing mice hydrodynamic injection. "Lateral Tail Vein Mouse" . A fine gauge needle should be used for this procedure. b. at 28-30 C) for up to 30 minutes, or by placing the tail in warm (30-35 C) water. Hydrodynamic injections were carried out as previously described [36] using a heat lamp for tail vein dilation, with the exception that sterile phosphate-buffered saline #20012 (Invitrogen, Carlsbad, CA) was used to dilute the DNA. . Typically, a manual tail vein injection consists of warming the mouse tail followed by securing the tail in the injector's hand and locating the tail vein by eye. . Place tail on heating pad and cover with hand iv. The lateral tail vein is usually used and 0.1 - 2 ml of blood can be obtained per sample depending on the size of the rat, sampling frequency and scientific justification. 1. 45-50C waterbath and/or heat lamp. Groman EV, Reinhardt CP. Then make sure that they can be shaded under the cage insert, such that they don't get too hot. After a tail vein injection of 3.7-7.4 MBq of 18F-FDG in 200 mL of PBS, a 7-min prone acquisition scan was performed approximately 60 min after injection. Prep the tail with 70% ethanol. 4-4: The mouse tail was extended and placed parallel to the table. Slightly rotate the tail to visualize the vein, and once located, disinfect the injection site using an alcohol swab. b. Prepare bone marrow from donors. tunnel restrainer). Tail Vein Injection. Mouse Intravenous Injections (Tail Vein) 1 - The tail vessels will be dilated by placing it in warm water (37-40 C) or placing the entire animal under a heat . place in a restrainer if conscious or anesthetize) and: i. Submerse the tail in warm water for 12 minutes ii. Solution must be buffered with sodium bicarbonate to a pH of 7.0-7.5. . Take a mouse, held by the tail, and guide it rear-first into the mouse restrainer. Skin Volume: <0.05ml Needle: <25 Gauge Rear Leg/Thigh Volume: <0.05ml . Intravenous injection into the mouse lateral tail vein can be a challenging . - Mouse cage with wire lid. 3. iii. Restrain the tail while occluding the vein with your nondominant hand. Title: Microsoft Word - SOP 7.12 - Tail Vein Injection in Mice.docx Author: Level of anesthesia is judged by loss of righting reflexes, loss of gulping reflex and loss of withdrawal response to toe pinch. View the tail vein sampling technique below. For image acquisition, the animal will be placed on plastic-backed absorbent paper to . Intravenous injections Intravenous injections are usually made into the dorsal tail vein. 4. For heating the tail of the mouse directly, restrain the mouse (i.e. Keep bevel up and parallel to the rest of the tail (it will be going into the tail at an angle because the tail is being held over your forefinger, if that makes sense). INJECTION: 1. Braintree Scientific has been serving the needs of the Life Science industry since 1980. Once the vein has been located, disinfect the site of injection and insert the needle at slight angle. We heated mice with a 50-W heat lamp until the lateral tail vein dilated. Tail vein injection in mice was performed as described by TE Gratsch et al. Traditionally, the needle insertion is performed manually by hand, where success rates depend on technical skill [].The most common vein used for needle insertion in rodents is the lateral tail vein, which for a typical lab mouse and rat is approximately 0.3 and 0.5 mm in diameter, respectively [7,8].Due to the small size of the vessels, oftentimes, they are difficult to localize and puncture. J Neurotrauma 2002;19:205-21. The mice are transferred to a holding device, (AIMS restrainer) . (B) Dilating the tail blood vessels with a heat lamp. 4-5: The needle was inserted into the tail vein from the distal end and the needle was held steady. Return to top Restrain the animal in a restraint device if animal is not anesthetized. Mouse: Grasp the tail at mid-length or at the distal . If the vein is not easily visualized or accessed, consider using another injection route, such as retro-orbital injection as described above. slowly (20-40 L/second) while watching for clearing of the . (E) Inserting the needle in the distal portion of the tail vein and injecting the DNA solution into the tail vein. $13.99 $ 13. (Tail may be immersed in . 4-6: The cell suspension was injected by gently pushing on the syringe's plunger. Lightly anesthetize the mouse with isoflurane gas. Visualize the lateral tail vein and insert needle parallel to the vein 2 to 4 mm into the lumen. Apply 70% Ethanol wipes to tail and turn 45o for a visible presentation of tail vein. Each unit comes with a restraining tube and illumination source. - Mouse restrainer. The present invention relates to a kind of multi-functional tail vein injection platform, described tail vein injection platform includes platform body, mouse cage, regulator, rat-tail bracket, temperature regulating device, light-guiding system device, magnifying glassDescribed mouse cage is fixed on the upper surface of platform bodyThe front end of the mouse cage is provided with . Mouse 20 1.10 - 1.40 11 - 14 l 90 - 105 l 110 - 140 l 25 1.37 - 1.75 14 - 18 l 102 - 131 l 140 - 180 l . You can also try ethanol (70%) and rub onto the tail before your injection. At the indicated time post injection, mice were given 200 l luciferin (15 mg/ml) intraperitoneally. In the case of femoral cut down, only two injections are possible due to making incisions in the veins limits repeated injections and the availability of only two femoral veins restricts this route. IV injections are most commonly performed in the tail veins. c. For a better visualization of the vein and dilation, warm up the vein to ~37 C using either a water bath or a heat lamp. If a warming box or incubator is used, its temperature should be monitored carefully. Following step IV.c.4, prick the vein perpendicular to the tail as close to the tail tip as possible. A heating pad, heat lamp, or hot water was used to dilate the tail veins for . With the bevel of the needle facing upward and the needle almost parallel to the vein, slide the needle into the tail vein. Heat the mice up first with the heat lamp until they exhibit whisker-grooming behavior. Shaver to remove mouse hair. Placing the mouse under a heat lamp, maximum wattage of 40 watt, for 3-5 minutes. Tail Nick 1. Inject the solution slowly so that it flows at a slow pace through the vein. Alternately, the tail can be warmed under a heat lamp. Ensure good hemostasis by applying pressure with a dry gauze pad; clotting agents or surgical glue may be used if needed. After anesthetic induction, the animals have their bladders emptied via a cred maneuver. . 3. needle info@signagen.com Injection Procedures: 1. ii. Try a heating pad to gently warm the tail and improve vasodilation. This procedure has the risk of needle stick or mouse bite injury - take appropriate care. Reagent setup Mice were then warmed by heat lamp for 30 min to recover from injection shock. 22. o The tail vein is difficult to find that's why mouse is often placed under a heat lamp to promote peripheral vasodilation o Suitable for large volumes. This may be done using a heating chamber, heating pad or a heating lamp, or dipping tail in warm water. N.B. (weight ranging 17-28 )grams. If no anesthesia is used, transfer mouse into the barrel of the plastic or brass restrainer, which restrains the animal while allowing access to the tail vein. Animals are anesthetized (another 2-3 minutes), then imaged. It is common practice to stimulate dilation of the tail veins in mice either by placing them in a warmer environment (e.g. The tail vein-illuminating ring comprises a light-emitting . Place the tail in large beaker filled with warm water (40C) for 30 seconds. Mouse Tail Vein Catheterization/Injection 5/6/08 . for catheter placement or tail vein injection of radiotracer. Here I introduce a special 180 LED illumination device to improve the visibility of the tail veins of black mice and report on the application of a Broome style restrainer as well as the selection. 4. (D) Immobilizing the mouse with a restraining device. They are. . Inject 150 ml cells (10 million) i.v. Wipe tail . Clean the tail with 70% alcohol. While holding the syringe in your right hand (if right-handed), carefully inject the needle into the vein, while still holding onto the vein so that it does not roll. Lateral Tail Vein Volume: <0.5ml Needle: <23 Gauge Back Scruff Volume: <5-10ml Needle: <20 Gauge; Hamster; Skin If an overhead heat lamp is used, extra care must be taken to prevent overheating the animal. which has a built-in heat source to help keep the animal warm. injection (refer to luciferin injection protocols). In a sterile culture hood, collect 300 l of cell . Arm Length: 2 spring loaded arms each 18" lengths Table Clamp : Standard "U" screw clamp expands to fit bench up to 2.25" deep Weight: 2 lbs. 3.7 MBq of FDG in 50-100 L of saline is . You should not feel any resistance if you insert the needle right into the vein. When doing an IV injection using the tail vein it is very convenient to have one of the commercial restraint devices . (iv) Placing the mouse in an incubator for 5 - 10 minutes set at 35oC. Injections can be made into either of these veins. 4. Stabilize the tail between the thumb and forefinger of the hand that will not be manipulating the syringe. Identifying the . With the torso in the main chamber of the restrainer, with the . oThe mouse should be anesthetized so that it remains still during . Technique: 1. 3. A heat lamp may be used if the water is not adequately warming the tail for the injection. Warm the bottom of the mouse tail under heat lamp for 10 s. v. Rapidly inject mouse via the tail vein (within 5 to 7 s) with syringe held at a 10 degree angle to the vein. warm water (~37C) or heat lamp (120 W bulb)) for 3-5 minutes. DONOR CELLS: (Need 10 million/mouse) 1. 2. LAB_030 Injections - Intravenous (IV) Tail Vein Injection in Mice and Rats Institutional author: UQ Biological Resources . Mice can be placed back into the original cage immediately after injection. Illuminated slot for easy tail vein injection, etc. Species: Mouse. Warming the tail causes the veins to dilate. The pack remains hot for at least 30 minutes. A mouse model of acute ischemic judged to have received an "excellent" injection, 3 received a spinal cord injury. 2. Best to heat the mouse for 2-3 min with a 200 W lamp to dilate the tail vein before injection. Intraperitoneal Injection : Intravenous Injection Subcutaneous Injection Mouse. The novel induction chamber for tail-vein injection of mice is made of a special polymethyl methacrylate (1cm; 60 120 50mm) with inlet and outlet adapters for fresh and waste anaesthetic gas. On day 0, animals are placed under a heat lamp for 4-5 minutes, and cells are injected i.v. Mouse Metabolic Phenotyping Centers MMPC Protocols 09/29/14 2 of 3 page(s) a. . Immerse frog in solution for 10-20 minutes. Lateral Tail Vein Injection in . Immobilize the tail with gentle traction. Place mice in clean cages and keep warm with a heat lamp for ~5 min with constant monitoring to induce vasodilation. A heating pad, heat lamp, or hot water was used to dilate the tail veins for injections. (A) Reagents and the apparatuses needed for the injection. The tail vein is easier to see in non-pigmented mice. saphenous vein); c) tail vein; d) retro-orbital; e) jugular vein; f) submental. - Heat lamp. QUESTIONS Ken Hays DLAR Training Coordinator 859-323-5697 H41C ken.hays@uky . o Warm water, a heat lamp, or hand warmers may be used to dilate the blood vessels. 1 It can be used to administer cells, chemical agents, infectious disease agents, drugs and any other reagent that needs a systemic delivery. The cap has . - Cotton gauze pads. Warming the mouse (or tail only) causes vasodilation and provides better vein visibility. Warming the mouse under a heat lamp helps to bring up the tail vein, but be careful not to heat stress the whole box as this may affect your results. Warm the tail by immersing it in warm water or placing the animal under a heat lamp. Retro-orbital injections in mice oThis technique is a useful alternative to tail vein injection. Mouse Tail Vein Injection (JK Lab 2006) Scale for weighing mice. If enough blood is needed, larger amounts should only be used, but care must be taken . Place mice to be injected (up to 6 mice) in cage and shine a heat lamp ~6 to 10 in. Blood withdrawal by cardiac puncture is . 10 Best Heat Lamp For Mouse Tail Vein Injection. To maintain the animal's body temperature, we use a heat lamp when the mouse is in the induction chamber and on the lab-built injection platform. There are two veins in the mouse's tail, one on the ventrum of the tail.
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