Application References. Stable for one year from the date of shipment. Calnexin was associated with glucosylated BACE457, whereas most of the protein associated with EDEM was nonglucosylated, as determined by JB-M treatment, . General Function Fibrillin-2: Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free . Molecular Cell Calnexin Regulates STAT3 Transcriptional Response Molecular Cell 51, 386-396, August 8, 2013 2013 Elsevier Inc. 387 In concert with ER lectin-like chaperones calnexin and calreticulin (CNX/CRT), ERp57 functions in virtually all folding stages from co-translation to post-translation, and thus plays a critical role in maintaining protein homeostasis, with direct implication for pathology. It was expressed in exosomes derived from all the eight cell lines at the molecular weight of 102 kDa (Fig. Nat Rev Drug Discov. The released cytosolic fragment of calnexin has a predicted molecular weight of 6.3 kDa, yet it migrates at an apparent molecular weight of 16 kDa in SDS-PAGE. Molecular weight: 67 kDa (predicted); 80-90 kDa (observed) Antibody target cellular localization: Endoplasmic reticulum. WB: Use at a dilution of 1/5000. The encoded protein.Show More. Furthermore, the anticalnexin antibody did not coprecipitate any 28-kDa protein from mock-infected cells. 2B), . Cnx was originally described as a protein transiently associated with partially folded major histocompatibility class I molecules in mice (Degen and Williams, 1991), and it was subsequently isolated in humans (Wada et al., 1991). Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. Studies indicate that calnexin associates with the . By Western blot, this antibody detects calnexin in human liver, skin, adrenal gland, heart, colon, testes and ovary extracts. (This dilution was sufficient for detection of Calnexin in 20g of HeLa heat shocked cell lysate). The Molecular Chaperone Calnexin Associates with the Vacuolar H+-ATPase from Oat Seedlings Xuhang Li,a Robert T. C. Su,b Hei-ti Hsu,c and Heven Szea1 aDepartment of Plant Biology, University of Maryland, College Park, Maryland 20742 bCenter for Scientific Review, National Institutes of Health, Bethesda, Maryland 20892-7840 No. WB: Use at a dilution of 1/2000 (this dilution was sufficient for detection of Calnexin in 20g of HeLa heat shocked cell lysate). This antibody recognizes Calnexin at the C-terminus. It does not detect calnexin without permeabilization and does not produce any non-specific staining without permeabilization. . Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. . . It is however important to note that only calnexin molecules with free cysteines can incorporate 3 H-palmitate. . Anti-Calnexin antibody [EPR21240] (ab241154) Research with confidence - consistent and reproducible results with every batch Long-term and scalable supply - powered by recombinant technology for fast production Success from the first experiment - confirmed specificity through extensive validation (E) Graph indicates RAB39B protein expression normalized on CALNEXIN. Anti-Calnexin Antibody, clone AF8 Anti-Calnexin, clone AF8, Cat. Influenza hemagglutinin, a well-characterized substrate associated with calnexin in complexes that sedimented at 5-5.5 S 20,W. 1. In our previous study, the calculated molecular weight of calnexin-t (M r 69 454) predicted from cDNA sequence data was much smaller than the 101-kDa migration size on SDS-PAGE . This large difference could be due to the high level of phosphorylation. Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. It specifically acts to retain unfolded or unassembled N-linked glycoproteins in the ER. Detects a band of approximately 90 kDa. No MSDS required. Preparation The antibody was purified by affinity chromatography. Molecular Weight 68; Add to Compare List. We have developed a method for the immunoaffinity purification of calnexin, an endoplasmic reticulum molecular chaperone, and analyzed the molecular weight of purified calnexin using matrix-assisted laser adsorption ionization time of flight mass spectrometry (MALDI TOF-MS). Human gene symbol: CANX. Calnexin Protein, Human, Recombinant (hFc Tag) 13929-H02H. After EGF treatment, our antibody against the C terminus of calnexin revealed not only the full-length protein (Figure 1A, upper blot) but also a 16 kDa fragment . Download Free PDF. Calreticulin also known as calregulin, CRP55, CaBP3, calsequestrin-like protein, and endoplasmic reticulum resident protein 60 (ERp60) is a protein that in humans is encoded by the CALR gene.. Calreticulin is a multifunctional soluble protein that binds Ca 2+ ions (a second messenger in signal transduction), rendering it inactive.The Ca 2+ is bound with low affinity, but high capacity, and can . Optimal dilutions/concentrations should be determined by the end user. Calnexin is a membrane-bound lectin and a molecular chaperone that binds newly synthesized glycoproteins in the endoplasmic reticulum (ER). The possibility that the precipitated protein was calnexin itself can also be ruled out, as calnexin has a molecular mass of 64 kDa . Thus, we conclude that calnexin interacted in a time-dependent manner with a virus-encoded protein of 28 kDa. To examine whether there is a direct interaction between calnexin and ERp57, advantage was taken of the large apparent molecular weight of TMC, which allowed its separation from ERp57 by gel filtration. Purified soluble calnexin and calnexin mutants were covalently bound to a sensor chip and ERp57 injected over the chip with mass changes recorded as described under "Materials and methods". The predicted molecular weight of this antibody is ∽67kDa; however, it may run on SDS-PAGE as an ∽90kDA protein. . STORAGE Store at 4 C, **DO NOT FREEZE**. Calnexin is a calcium-binding protein embedded in the ER membrane that retains the newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (3-5). Calnexin is a calcium-binding protein embedded in the ER membrane that retains the newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (3-5). GeneTex. Cell Signaling Technology. 2001, Biochemical Journal. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. SwissProt: P27824. Data are presented as mean SEM . Optimal dilutions/concentrations should be determined by the end user. Kind Protein Organism Humans Pharmacological action. 5. Thus, a small amount of ERp57 was mixed with an excess of TMC and examined by size exclusion chromatography. Cell Signaling Technology's Calnexin Antibody is a Rabbit Polyclonal antibody. Fig. ICC: Use at a dilution of 1/200. For calnexin-substrate complexes the S-values ranged between 3.5-8 S 20,W, the size in-creasing with the molecular weight of the substrate. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. Influenza hemagglutinin, a well-characterized substrate associated with calnexin in complexes that sedimented at 5-5.5 S 20,W. Calregulin has both low- and high-affinity calcium-binding sites. (predicted molecular weight: 71 kDa). 2a), indicating successful isolation of exosomes. Overington JP, Al-Lazikani B, Hopkins AL: How many drug targets are there? This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER ( PubMed: 7876246 ). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (5). Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Nat Rev Drug Discov. E.Chevet et al. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. The results support our hypothesis that both calnexin and BiP act as molecular chaperones in the folding and assembly of newly synthesized V 1 V o-ATPases at the endoplasmic reticulum. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. Thus, the bulk of cellular calnexin (>90%) is 'silent' in the 3 H-palmitate incorporation assay and we are monitoring the incorporation of 3 H-palmitate in the remaining population. MWM: molecular weight marker of protein ladder. . """. Related Products: . It acts as a chap . Molecular weight marker positions (kDa) are indicated on the left. The difference in the protein molecular weight was minor, disappeared at later chase times (Fig. Serves as a negative control to assess the purity of isolated exosome extracts. 13 January 2016 | British Journal of Nutrition, Vol. Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). (A) Two-dimensional tryptic phosphopeptide mapping of phosphorylated calnexin fromMDCK cells after in vivo radiolabeling, calnexin immunoprecipitation and trypsin digestion (map 1). (predicted molecular weight: 71 kDa). 115, No. Recombinant Calnexin Protein Feature . MABF2067, is a mouse monoclonal antibody that detects calnexin in human cells and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded . Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic . . Molecular weight (Da) 65395.57 Theoretical pI . Detects a band of approximately 90 kDa. ICC: Use at a dilution of 1/200. Calnexin Molecular Weight 67567.695 Da. For calnexin-substrate complexes the S-values ranged between 3.5-8 S 20,W, the size increasing with the molecular weight of the substrate. Anti-Calnexin precursor antibody produced in rabbit, Anti-CNX, Anti-CANX, Anti-Calnexin -NT, Anti-P90, Anti-IP90, Anti-Calnexin-CT (Purified) Compare It was also possible that this higher For calnexin-substrate complexes the S-values ranged between 3.5-8 S20,W, the size increasing with the molecular weight of the substrate. Association of calnexin with invariant chain trimers. 4.46 Calnexin Protein Interaction . CFTR and calnexin (CNX) were detected by western blotting using mouse monoclonal antibody 596 for CFTR or rabbit polyclonal antibody SPA860 (Stressgen) for calnexin (as indicated on the right). Demonstrated to react with Human, Mouse, and Rat. References. Calnexin is a type I membrane protein that inter- acts with newly synthesized glycoproteins in the endoplasmic reticulum. Fibrillin-2. The molecular basis of oculocutaneous albinism type 1 (OCA1): sorting failure and degradation of mutant tyrosinases results in a lack of pigmentation. Calnexin is highly abundant in ER and is frequently used as an ER marker. Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. This gene encodes a member of the calnexin family of molecular chaperones. Richard Spritz. The low-molecular-weight part was transferred for 7 min. OR. is a 67 kDa integral protein of the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassem- bled protein subunits in the endoplasmic reticulum. Boster Bio Anti-Calnexin/CANX Antibody Picoband catalog # A03372-2. IP: Use at a dilution of 1/100. . 2006 Dec;5(12):993-6. Calnexin is a chaperone, characterized by assisting protein folding and quality control, ensuring that only properly folded and assembled proteins proceed further along the secretory pathway. The 3175-bp full-length cDNA of EsCnx encodes a polypeptide of 598 amino acids; this peptide possesses a predicted molecular weight of 68 kDa and a theoretical isoelectric point of 4.50 (Fig. Immunogen: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is . Concentration 0.5 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2C and 8C. Knockout; . Cell Signaling . CALNEXIN was used as housekeeping gene. For calnexin-substrate complexes the S-values ranged between 3.5-8 S 20,W, the size increasing with the molecular weight of the substrate. Remove the permeabilization buffer, and wash the wells twice with 100 l of 1 PBS. Cell/tissue expression: All cells . Comparison with two-dimensional analysis of calnexin tryptic phosphopeptides from EDTA-stripped canine pancreatic rough microsomes (EsRM) after . Compare Anti-Calnexin antibody [C3], C-term GTX109669 from GeneTex on Biocompare.com . Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin Molecular Weight 67567.695 Da. Calnexin, also referred to as IP90, p88 and p90, is an . This antibody has been shown to work in applications such as: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot. In-fluenza hemagglutinin, a well-characterized substrate associated with calnexin in complexes that sedimented at 5-5.5 S 20,W This antibody is not recommended for human kidney or mouse liver lysates in Western blot applications. 2. 13929-H02H. UniProt: Calnexin ; Details9. Weight loss-induced stress in subcutaneous adipose tissue is related to weight regain. The bar graphs are the R max values of wild-type calnexin and calnexin mutants normalized for molecular weight and valence. Validated antibody for detection of endoplasmic reticulum protein calnexin by fluorescent western blot. The majority of stable complexes extracted from cells, appeared to contain a . Synthetic peptide corresponding to Human Calnexin aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. This is due mainly to negative charges at the C-terminus of the protein, which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility. Calnexin (Cnx) is a membrane-bound lectin chaperone of the endoplasmic reticulum. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Predicted to react with Chimpanzee, Rhesus Macaque, Equine, Porcine, and Bovine based on 100% sequence homology. The antibody SPC-108 is an excellent antibody for immuofluorescent detection of calnexin in the fixed (1% formaldehyde) human tumor cells. In order to rule out extravesicular protein contamination, calnexin, an endoplasmic reticulum protein aiding in proper protein folding , was used to investigate the purity of exosomes . The kit can be used for measuring the relative amounts of Calnexin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. Cnx is a highly conserved chaperone widely distributed in eukaryotic organisms. . As the C-terminus of calnexin E exhibits an acidic domain with a highly negative charge, the apparent . Read the full review on Biocompare.com. (50 gm fresh weight) were homogenized in a cold room at 4C with a mortar and pestle in 100 mL of a medium containing 50 mM Hepes-bis-Tris propane . siRNA or . The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein . Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. Molecular Weight of Calnexin: 90 kDa. L1-L5 OsCNX-OE lines, VC vector control, M molecular weight marker. This gene encodes a member of the calnexin family of molecular chaperones. For optimal transfer to monitor calnexin cleavage, the gel was cut in two, and the high-molecular-weight half was transferred with iBlot for 15 min. Non-hazardous. Calregulin has both low- and high-affinity calcium-binding sites. The molecular chaperone calnexin binds Glc1Man9GlcNAc2 oligosaccharide as an initial step in recognizing unfolded glycoproteins.. J. Biol. Imming P, Sinning C, Meyer A: Drugs, their targets and the nature and number of drug targets. Entrez gene ID: 821. Interacts with the DNA-binding domain of NR3C1 . Unigene: 567968. The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (5). In this study, a novel Cnx homologue from the obscure puffer Takifugu obscurus was characterized, tentatively named ToCnx. 2006 Oct;5(10):821-34. . GeneTex, Inc. 2456 Alton . Anti-Calnexin antibody [EPR3633 (2)] - ER Membrane Marker (ab133615) Research with confidence - consistent and reproducible results with every batch Long-term and scalable supply - powered by recombinant technology for fast production Success from the first experiment - confirmed specificity through extensive validation Notably, as was the case without chaperones, higher molecular weight . This distinction between folded and unfolded CFTR also involves a role for ER lectins such as calnexin, which is dependent on CFTR glycan moieties. . No. The first attempts of using low molecular weight compounds to repair CFTR were targeted at folding/trafficking mutations and aimed at correcting the folding process either acting directly at . 1SA). and it contained an open reading frame encoding a polypeptide of 600 amino acid residues with a calculated molecular weight of 67.5 kDa . Calnexin Protein Molecular Weight & PI . The molecular chaperone calnexin plays key roles in the translocation of nascent polypeptides and in the folding and quality control of newly synthesized proteins (Bukau et al., . Phosphorylation of calnexin by CK2 and ERK-1. Add 100 l of -20C chilled Perm Buffer III (Cat. Continue Reading. It consists of a large (50 kDa) N-terminal calcium-binding lumenal domain, a single transmembrane helix and a short (90 residues), acidic cytoplasmic tail. (C) The channel properties of unglycosylated CFTR are similar to wild-type CFTR. Full size image. the unoccupied calnexin behaved as a monomer sedi-menting at 3.5 S 20,W. The apparent molecular weight of recombinant calnexin E was approximately 35 kDa . Calreticulin is an ER-resident chaperone, and a model for class I assembly in human cell lines has been proposed in which calreticulin replaces calnexin upon binding of 2 m to the calnexin-HC . [6] For the western blotting of calnexin, the samples were run on 15% gels unless specified otherwise. This gene encodes a member of the calnexin family of molecular chaperones. 4. Tested in ELISA, IF, IHC, ICC, WB applications. No. Calnexin (CNX) is an integral membrane protein of endoplasmic reticulum (ER) and is a critical component of ER quality control machinery. in ADAMTS13 CUB domains cause abnormal pre-mRNA splicing and defective secretion of ADAMTS13 Journal of cellular and molecular medicine April 1, 2020 Jiang Y, Huang D, Kondo Y . Protocols Standard Protocols Click here to view our Standard Protocols Publications All (14) WB (10) IF (4) b. Calnexin; CANX; IP90; Major histocompatibility complex class I antigen-binding protein p88. Molecular Weight 90; Add to Compare List. One had a molecular weight con- sistent with a trimer (13) and the other a potential hexamer (16) of invariant chain (22). Date published: 2021-04-16. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted. In Journal of Molecular Medicine (Berlin, Germany) on 9 August 2022 by van der Kooij, M. A., Rojas-Charry, L., et al.. Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Add 100 l of 0.1% Triton X-100 to each well and incubate for 5 minutes at RT. The predicted molecular weight of this antibody is ~67kDa; however, it may run on SDS-PAGE as an ~90kDA protein. (CFBE) Stron and specific band at teh expected molecular weight. Human Calnexin recombinant protein (161-410 a.a.) expressed in E. coli . Molecular weight: 67 kDa (predicted); 80-90 kDa (observed) . molecular-weight proteins. Molecular Requirements for Calnexin Association 1 2 3 p43/41 200 97 69 46 200 97 69 46 30 14 FIG. Positive Controls: A-431 whole cell lysate: sc-2201, HeLa whole cell lysate: sc-2200 or Calnexin (h3): 293T Lysate: sc-170477. Calnexin Antibody (E-10) is a monoclonal anti-CANX antibody that detects m, r, and h Calnexin by WB, IP, IF, IHC(P) and ELISA. Cited in 53 publications. 558050) or 90% methanol to each well and incubate for 5 minutes at RT. Calreticulin is an endoplasmic reticulum resident protein of approximately 46kDa molecular weight with multiple functions, including control of cellular adhesiveness, gene expression, calcium homeostasis regulation, and molecular chaperoning.23 . After cross-linking, several high-molecular-weight products were observed, most strikingly two prominent bands with identical mobility to the cross-linking products detected by the calreticulin antisera . IP: Use at a dilution of 1/100. (Peptide available as ab23379) Validations. a. Calnexin belongs among chaperones, which are characterized by their main function of assisting protein folding . The Calnexin Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Calnexin protein expression profile in cells. Choose a Store . Influenza hemagglutinin, a well-characterized substrate . Calnexin, encoded by the CANX gene, is a member of the calnexin family of molecular chaperones. Calnexin (CNX) is a 90kDa integral protein of the endoplasmic reticulum (ER). - Find MSDS or SDS, a COA, data sheets and more information. These results suggest that the 101-kDa protein is a phosphorylated form. . High Purity . Calreticulin is a 60 kDa homologue of calnexin, and like calnexin contains a globular glycan .
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